Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ded504b18a27b7d519fdb42769fa0b2 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24251 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32451 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-16051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2730-10151 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D71-32 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate |
1998-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2011-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bde5005ae42556c5b894511fd4a70ed4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ed1fac3a37ce30e022a1475c0601dca |
publicationDate |
2011-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2296733-C |
titleOfInvention |
Methods for purifying viral nucleic acids using a hydrophilic polyvinylidine fluoride (pvdf) membrane |
abstract |
The invention relates to a method for purification of viral RNA from a biological sample. The method involves lysing the virus envelope to liberate the RNA and passing the lysate through a porous hydrophilic polyvinylidine fluoride (PVDF) filter to capture the viral RNA. The filter with bound RNA is then washed to remove proteins, lipids and other contaminants. The RNA is released from the filter using a low ionic strength ribonuclease (RNase) free solution to form a solution containing purified viral RNA. From this solution the RNA is recovered. The invention is also compatible with purification of nucleic acids from other types of samples. |
priorityDate |
1997-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |