http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2293777-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_857f8336175d4a3144facf2e9ad3998b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-547 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 1998-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_540ce400a5bd04244323186295af15a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b84991ad53dc75f5624687359afb3d26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b77704e7b6cafd57eea21830630c927 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc1d5f47a49250df2b1908db08823242 |
| publicationDate | 1999-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2293777-A1 |
| titleOfInvention | Methods for covalent immobilisation of biomolecules to a carrier by means of a his-tag |
| abstract | The present invention relates to methods for covalent immobilisation of biomolecules to carriers and membranes, wherein the presence of a His-tag is exploited, and wherein the amino acid residues that comprise said His-tag are directly involved in the covalent bond. The present invention also provides several strategies that further augment the probability of covalent immobilisation through said His-tags, such as improving the presentation of said His-tag, choosing the appropriate reaction conditions such as pH, temperature, concentration of reagent and kinetics, increasing contact between His-tag and reactive groups of said carrier or membrane, by for instance the use of IDA or anti-His antibodies or increasing the hydrophobicity of the membrane, or shielding the rest of the biomolecule from reaction by for instance increasing the hydrophobicity of said carrier or membrane or addition of substrate or competitors or blocking otherwise reactive groups, or by choosing chemical reactions that have a high selectivity for histidine residues. A carrier can also be another biomolecule. The present invention thus also relates to a method that allows covalent cross-linking between identical or different biomolecules. When such biomolecules have a natural tendency to interact with each other to form homo- or heterodimers, a strategy of increasing contact between the reactive groups (two His-tags or one His-tag and another reactive group) can be exploited. The present invention also relates to a method of providing a simultaneous and universal system for detection of biomolecules through said His-tag. |
| priorityDate | 1997-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 154.