http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2292897-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a3e7bb7512865a94b3991f488c20671 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8a033d7bf2fc58cc11ccb02f8170d8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b8641b7621c06c32b5d1aebcedf6bb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b73e5863a2325f398bacdeaabdaefd46 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 1998-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2007-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb776389323cc593787c5b0e5bfe4ec3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4e7cc884b42799a87ef3060a38043dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_411dbbb87234bb79d3df4f0bef250a00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b01ba78c67c398510b7e5eaab2833cc9 |
| publicationDate | 2007-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2292897-C |
| titleOfInvention | Recombinant construct for enhancement of gene expression in plants |
| abstract | The present inventions focuses on the super-expression of foreign genes in transgenic cells by combining within a single cDNA construct and respective RNA transcript, several trans- and cis-acting genetic elements of viral origin which will act in concert to trigger the following functional events: a) the primary chimeric continuous RNA transcript is produced by the transformed cells from plant-expressible promoter (35S promoter); b) RNA replicase produced by direct translation of the 5'-proximal gene of a single continuous primary transcript will synthesize secondary monocistronic (or dicistronic) mRNA as a result of the transcription from sgPr sequence. Expression of the 5'-proximal gene of these mRNAs will be enhanced by the .alpha..beta.-leader. Translation of the 5'-distal gene of dicistronic mRNA will be promoted by internal ribosome entry site (IRES) sequence derived from crTMV tobamovirus mentioned above; c) it is probable that at least part of RNA transcripts originated from sgPr will include at their 3'-end the minus copy of RNA replicase gene and genomic promoter for plus-RNA synthesis. It can be expected that RNA replicase produced in transgenic cell will bind with the 3'-terminal sequence of this RNA (genomic promoter) producing upon transcription the RNA molecules carrying the plus-polarity replicase gene at the 5'-end. Translation of these mRNAs will result in production of additional replicase in transgenic plant. |
| priorityDate | 1997-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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