Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8387699b0be46cdfddfb7ffe82cf666f |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-76 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
1999-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a1b0ed17b3cf2f8954fbdbd581ca34f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90d05e855d5f398d4fde88b4cf8e35cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb2fb61470108a13bfe0932e6769cbe8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc454666426503db9b397e4eb21be568 |
publicationDate |
2000-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2292889-A1 |
titleOfInvention |
Process for the separation of glycosylated and nonglycosylated proteins |
abstract |
A process for the partial or complete separation of glycosylated and nonglycosylated proteins is described, in which a) a triazine dye immobilized on a matrix is incubated with a mixture of glycosylated and nonglycosylated proteins, b) the matrix is then washed to remove the unbound proteins, and c) the proteins are eluted by means of a stepwise or continuous increase in the ionic strength or in the pH, nonglycosylated proteins and proteins having an increasing degree of glycosylation being collected separately from one another in the eluate fractions obtained. By means of the use of this process it is possible, for example, to prepare a human albumin which is free of glycoside bonds and is expressed in yeast cells. |
priorityDate |
1998-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |