http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2282737-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efaece3f57d915a44fb0784b21caeab8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c0a74137206e84c1a26fd75f65cca33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9599ac43b874871bf69957eb3e714398 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c64001e0fdbe7e37937b2d20c8b6ca4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_747ac117d98070272f27402723c84ea0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14f90b20d40b8d65036b039aa6bc5370 |
| publicationDate | 1998-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2282737-A1 |
| titleOfInvention | Methods for cleaving dna with nucleotide integrases |
| abstract | The present invention provides methods, using a nucleotide integrase, for cleaving nucleic acids substrates at specific sites and inserting a nucleic acid molecule into the cleaved substrate. The method of cleaving one strand of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on the one strand of the substrate and a group II-intron encoded protein which binds to a first sequence element of the substrate. The method of cleaving both strands of a double-stranded DNA substrate comprises providing a nucleotide integrase comprising a group II-intron RNA having two hybridizing sequences capable of hybridizing with two intron RNA binding sequences on one strand of the substrate and a group II-intron encoded protein capable of binding to first and second sequence elements in the recognition site of the substrate. The method of cleaving a single-stranded nucleic acid substrate comprises providing an integrase having two hybridizing sequences capable of hybridizing with two intron RNA-binding sequences of the substrate and a group II-intron encoded protein. |
| priorityDate | 1997-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 295.