http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2277165-C
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D21-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D61-14 |
filingDate | 1998-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2004-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39657061c2b33e346baccf9872c4c07d |
publicationDate | 2004-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2277165-C |
titleOfInvention | Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation |
abstract | The invention concerns a method of purifying and/or concentrating nucleic acids in a solution, the solution containing nucleic acid being guided tangentially past one or a plurality of semipermable membranes, such that the nucleic acid molecules are retained by the membranes and substances having a lower molecular weight can pass through th e membranes and/or be adsorbed thereat, so that a purified and/or concentrated nucleic acid solution is obtained. The same method is carried out to separate endotoxins from a nucleic acid preparation. The invention further concerns the use of a cross-flow filtration system for purifying and/or concentrating nucleic acids in a solution and for separating endotoxins from a nucleic acid preparation. The invention also concerns the use of the nucleic acids purified and/or concentrated by the cross-flow filtration system for cloning, transformation, transfectio n and microinjection into cells, for use in gene therapy processes, DNA vaccination and/or for polymerase chain reaction (PCR). |
priorityDate | 1997-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.