| abstract |
Disclosed are 3'-deoxyribonucleotide derivatives represented by the following general formula [I]: Q-V-(CH2)n-NH-R [I] wherein Q represents a 3'-deoxyribonucleotide residue, n represents an integer not less than 4, V represents -C~C- or -CH~CH-, and R represents a fluorescent group. The above 3'-deoxyribonucleotide derivatives are derivatives with improved rates for incorporation using RNA polymerases, which are useful as terminators in the DNA sequence determination methods utilizing RNA polymerases. |