http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2252801-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed76644a34091fdf4d3065dd19b50c44 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1997-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef36bc4edc32e5dcbed26d9a841fe8f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5216bcddce4bbfc3f33879c9c19925d0 |
| publicationDate | 1997-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2252801-A1 |
| titleOfInvention | Subtractive amplification |
| abstract | A method is provided for preferentially amplifying target RNA relative to nontarget RNA in a sample of tester RNA. According to the method, a sample of tester RNA is contacted with driver sequences which are complementary to the non-target RNA under conditions where the driver sequences hybridize to the non-target RNA. A nucleic acid primer is then extended using the target RNA as a template, forming a DNA template complementary to some or all of the target RNA. The DNA template formed using the target RNA as template is then rendered single-stranded to enable the hybridization of the DNA template to a promoter template. The DNA template is then extended using the promoter template to form an extended DNA template comprising a functional double-stranded promoter. Multiple copies of the target RNA sequence can now be transcribed from the extended DNA template through recognition of the contained functional double-stranded promoter by RNA polymerase. |
| priorityDate | 1996-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 352.