http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2252412-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49310188df8dc04b3708491df863459f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8212 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8209 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 1997-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be35c94828be9fab27b0ef1fd1a8747b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49f3b86ed853c97a5647e2418268fe63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56a60cf57484564d96210cc2733a960c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04257e287ea7e984adec376b8fab1fda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64a072c04535f19633058b8123065ea1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d65fef6953e928b1782cefdd89538c27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d08728043f2213ff93d7f9bf93729c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26d975766e2d30527a392033eb14d790 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15c9ba8cef1029573c25d57771442886 |
| publicationDate | 1997-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2252412-A1 |
| titleOfInvention | Use of the green fluorescent protein as a screenable marker for plant transformation |
| abstract | A method for the production of transgenic plants is provided in which a vector carrying a gene encoding the green fluorescent protein is introduced into cells, the cells are screened for the protein and transformed cells are selected and regenerated. The cellular toxicity of the green fluorescent protein is circumvented by regulating expression of the gene encoding the protein or directing the protein to a subcellular compartment where it is not toxic to the cell. DNA constructs are provided for cell transformation in which the expression of a gene encoding the green fluorescent protein is placed under the control of an inducible promoter. In addition, DNA constructs are provided in which a nucleotide sequence encoding the green fluorescent protein is operably linked to a signal sequence which directs the expressed protein to a subcellular compartment where the protein is not toxic to the cell. Oxidative stress to plant cells transformed with GFP also can be ameliorated by transforming cells with an expression vector comprising genes encoding GFP and an oxygen scavenger enzyme such as superoxide dismutase. The toxicity of GFP in transformed plants can be eliminated by excising the screenable marker gene following detection of transformed cells or sectors. The FLP/FRT system is used in conjunction with GFP as a visible marker for transformation and FRT excision. A nucleotide sequence optimized for expression of the green fluorescent protein in plants is also provided. |
| priorityDate | 1996-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.