| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f263177549abb86bdd90d541968fca12 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H23-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate |
1996-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2009-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8d0449740b68034d317c4c435d721e5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a5eb80df023af8c3508382c1f84b3d5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0095fde85d1fec4ededd22c2757e47c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b526490fd114b9731267718cd9064acf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ebb1fffc75b489a17c4004d71f4e5cb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_705f7aea96a1f760996a2c361c165a0d |
| publicationDate |
2009-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2245666-C |
| titleOfInvention |
Solid phase synthesis of oligonucleotide n3'.fwdarw.p5' phosphoramidates |
| abstract |
The invention provides a method of synthesizing oligonucleotide N3'.fwdarw.P 5' phosphoramidates using an amine-exchange reaction of phosphoramidites in which a deprotected 3'-amino group of a solid phase supported oligonucleotide chain is exchanged for the amino portion of a 5'-phosphoramidite of an incoming monomer which has a protected 3'-amin o group. The resulting internucleotide phosphoramidite linkage is then oxidized to form a stable protected phosphoramidate linkage. The method of the invention greatly improves product yields and reduces reagent usage over currently available methods for synthesizing the above class of compound. |
| priorityDate |
1996-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |