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filingDate 1998-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecaf48112f16a6954323c705c2c76972
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c4c7d0d658a8a3b20d019d9f88da634
publicationDate 2000-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2242116-A1
titleOfInvention Method for genetic engineering of disease resistance using the dr206 class of proteins
abstract To identify genes effective against the blackleg fungus Leptosphaeria maculans, we have transformed canola (brassica napus) with four pea (Pisum sativum) genes under constitutive control by the CaMV 35S promoter: PR10.1, chitinase, Drr206 and defensin. Transgenic lines containing single copy T-DNA insertions for each gene were screened for both seedling (cotyledonary) and adult plant resistance. Lines for which pea Drr206 or defensin mRNA was expressed also showed decreased disease scores when inoculated with L. maculans or Sclerotinia sclerotiorum, compared to non-expressing transgenic lines. For PR10 and chitinase transgenics, there was little or no enhancement of resistance. Furthermore, resistance to L. maculans co-segregated with Drr206 and defensin transgenes. Extracts from Drr206 and defensin transgenic plants inhibited fungal growth in-vitro. Drr206 transgenic plants also demonstrated decreased hyphal growth at inoculation sites, and evidence of a hypersensitive response.
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