| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6115b7f4561db088f26a575e9339fed0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9bf7439808d578ab8c3698f7560fe4ab |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-16 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K36-18 |
| filingDate |
1996-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2008-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68d9ab7a82d963615945f5e5ea5dbe2e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac69333bff106b4347b25ba8cc0d252d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6296fdf9f045f29259300490e745b69f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41ff6149c637c143bc56adcf541aace3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31fa18fe176cc5b21b8d23984adf732e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f9dc4d250bb090affc49bd6792f657d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e9a1de68cd6f0eff234c8a0a204f51c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0dd19eeaf8ee4f6d94b6b58e1ea3eff5 |
| publicationDate |
2008-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2241918-C |
| titleOfInvention |
Peanut allergens and methods |
| abstract |
Peanuts are a common cause of food hypersensitivity reactions. The sera of 10 patients who had atopic dermatitis and a positive double-blind placebo-controlled food challenge to peanut were used to investigate the major allergens of peanut. Crude Florunner extracts were fractionated by anion-exchange chromatography using a step gradient (limit buffer, 0.05M BisTris/1.5M NaCl). A protein peak (OD 280) which eluted at 10t NaCl and demonstrated intense IgE-binding was further analyzed by two-dimensional SDS-PAGE/immunoblot analysis. The majority of this fraction is a protein which has a molecular weight of 17 kD and a pI of 5.2. Sequencing data from the N-terminus revealed the following initial 9 amino acids: (*)-Q-Q-(*)-E-L-Q-D-L. Based on IgE-binding activity and no known amino acid sequence identity to other allergens, this allergen is designated Ara h II. Ara h II may be used to detect and quantify peanut allergens in foodstuffs. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using Ara h I specific oligo-nucleotides and polymerase chain reaction technology. The Ara h I clone identified a 2.3 kb mRNA species on a Northern blot containing peanut poly A+RNA. DNA sequence analysis of the cloned inserts revealed that the Ara h I allergen has significant homology with the vicilin seed storage protein family found in most higher plants. The isolation of the Ara h I clones allowed the synthesis of this protein in E. coli cells and subsequent recognition of this recombinant protein in immunoblot analysis using serum IgE from patients with peanut hypersensitivity. |
| priorityDate |
1995-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |