http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2236711-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb75b0b19820e3833ebdcb31603b242b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1996-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_610c1e9f76543a368fd04267c3de40bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_985fc4f71522d8eb1cd4fbc6d045966b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2899d2667de29cfcb58f7f3f9917b5fb |
| publicationDate | 1997-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2236711-A1 |
| titleOfInvention | Method for the simultaneous detection of polygenes |
| abstract | A method for the simultaneous detection of polygenes involving the following steps (a) to (f): (a) the step of converting an mRNA into a cDNA by using primers for reverse transcription having the following characteristics (1) to (3): (1) having in the 3'-terminal side of the oligo dT an anchoring part represented by 5'-M(N)q-3' (wherein M represents a base selected from among A, G and C; N represents a base selected from among A, G, C and T; and q is 0 or 1); (2) having in the 5'-terminal side of the oligo dT a site for forming a solid phase; and (3) having an enzyme cleavage site between the 5'-terminus of the oligo dT and the site for forming a solid phase; (b) the step of cleaving the cDNA with a restriction enzyme; (c) the step of labeling the restriction enzyme cleavage site of the cDNA; (d) the step of forming a solid phase of the labeled cDNA fragment and recovering the same; (e) the step of cleaving the enzyme cleavage site in the 5'-terminus of the reverse transcription primer with an enzyme to thereby give a solubilized and labeled cDNA fragment; and (f) the step of subjecting the labeled cDNA fragment to two-dimensional gel electrophoresis. |
| priorityDate | 1995-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.