http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2226405-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8090a5ea39f9b1b1658845081b6952ab |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44704 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44773 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44791 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82B1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1996-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d67623630176d64ee6ee1a2b22782d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77b42473ca70021dbb14dce007b2e9ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9f6e39f0510ef464427fa44c4271745 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f23a8cbcebd27e44029d8a186bde4e06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_109a26bc120e6aadb8f831030beb73af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57f1897de86294492853f9cf99893934 |
| publicationDate | 1996-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2226405-A1 |
| titleOfInvention | Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same |
| abstract | Separation matrices useful in the formation of solid-state mm- to cm-scale devices for the rapid, high-resolution separation of single-stranded DNA ladder bands generated by the Sanger dideoxy- or Maxam/Gilbert chemical DNA sequencing procedures are formed from a solid support (1) having a plurality of posts (4) disposed on a first major surface thereof to form an obstacle course of posts (4) and pores (5). The posts are arranged in a regular X, Y array and are separated one from another by a distance of 100 nm or less, preferably 10 to 30 nm, and are optionally separated into lanes 2. The separation matrix can be manufactured by first forming a mold, preferably a reusable mold using lithography techniques. The mold is the reverse of the desired pattern of posts and pores of the obstacle course, and is used for casting the obstacle course. The cast obstacle course is then fused to a solid support and separated from the mold. Alternatively, the separation matrix can be formed from a polymer which undergoes specific and quantifiable swelling in the presence of a selected chemical compound. In this case, the matrix is cast on a mold in a conventional manner with a spacing between posts greater than the desired final spacing of 100 nm or less. For use, a buffer solution saturated with the specific chemical agent that controls swelling is added, causing the posts to swell to a defined amount to achieve the desired separation. |
| priorityDate | 1995-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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