abstract |
The present invention relates to a method for quantitative determination of l, 5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1, 5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of l, 5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1, 5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1, 5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture. |