http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2203758-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e0c9decd89e0ec3c2815631e084dd40 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2337-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 1995-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4c12fa9d1fa728669a717fc5f6661fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d95c78e155718f8ba50061b740956bfb |
| publicationDate | 1996-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2203758-A1 |
| titleOfInvention | Compositions for the detection of proteases in biological samples and methods of use thereof |
| abstract | The present invention provides for novel reagents whose fluorescence increases in the presence of specific proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. These protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections, because of their high fluorescence signal in the visible wavelengths. Figure 1 shows an HPLC analysis of the D-NorFES-A protease indicator comprising 5'-carboxytetramethylrhodamine (C2211) as a donor fluorophore and rhodamine X acetamide (R492) as an acceptor fluorophore so that Figure 1 (A) illustrates HPLC of the intact indicator molecule before the addition of elastase; Figure 1 (B) illustrates HPLC where both fluorophores absorb after the addition of elastase and Figure 1(C) illustrates HPLC after the addition of elastase where rhodamine X acetamide (R492) absorbs maximally. This invention also provides for methods of detecting protease activity in situ in frozen sections. |
| priorityDate | 1994-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.