http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2197479-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_16b2cf0886caade9971000b1fe60e6c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aa1e75d3a9aac3d099d2608e1437a57 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20 |
filingDate | 1995-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fc66ac2f6fd68d6fee59e0012adc95f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2264d1ecf95b856dfc4727821f77fe4f |
publicationDate | 1996-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2197479-A1 |
titleOfInvention | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
abstract | A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. The figure is a diagram of enzymatic synthesis of an oligonucleotide using a template independent polymerase and a nucleoside 5'-triphosphate having a removable blocking moiety at its 3' position. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed. |
priorityDate | 1994-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 471.