http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2194350-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d3e9d77ff72f5282325b4e142dfa5b4c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-06 |
| filingDate | 1995-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9c8e9f78badd7d7ec3c6eaaf267d695 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b208d387e27f5e5518577d632657d419 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf9ad6b2d8e0a3687bb581b64fa1f830 |
| publicationDate | 1996-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2194350-A1 |
| titleOfInvention | Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography |
| abstract | The present invention provides improved methods for separating and purifying oligonucleotide phosphorothioates. In certain aspects of the invention, oligonucleotide phosphorothioates are purified by a method comprising applying a mixture of the oligonucleotide to a column containing a DEAE-5PW anion ionexchange chromatography resin and eluting with an elution buffer having a concentration of sodium chloride of from about 0-2M. In other aspects of the invention, oligonucleotide phosphorothioates are purified by a method comprising applying a mixture of the oligonucleotide to a column containing a phenyl-sepharose fast flow chromatography resin or a phenyl-5PW chromatography resin and eluting with an elution buffer substantially free of salts. The methods of the present invention provide the ability to purify ammoniacal solutions of oligonucleotides cheaply, quickly, and on a large, process scale. |
| priorityDate | 1994-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.