abstract |
A porcine EG cell line has been established which is derived from porcine primordial germ cells, has a multipotency or totipotency like that of an embryonic stem cell, and can be subcultured on feeder cells. The line is reactive with a monoclonal antibody 4C9 prepared by using a mouse embryonic carcinoma cell F9 strain as the antigen. As the established EG cell line can be readily subjected to gene manipulation under culture, in particular, gene targeting, it is easy to produce a variety of swine by gene manipulation. |