http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2180540-C
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-882 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-315 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M25-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-14 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
| filingDate | 1995-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c20b7accc7804f68b1356303e5f6e0fc |
| publicationDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2180540-C |
| titleOfInvention | Method for detecting staphylococci |
| abstract | The present invention exploits the herein first reported, empirical observat ion that even though staphylococci produce the enzyme, .beta.-glucosidase, this genus of bacteria is not able to produce a metaboli te that will enzymatically react with 5-bromo-4-chloro-3-indolyl-.beta.-D- glucopyranoside, a substrate commonly used to detect the presence of .beta.- glucosidase produced by other bacteria. In view of this unexpected observation, one embodiment of the present invention includes a selective me dium containing inhibitors to enhance staphylococci growth as well as a first glucopyranoside substrate. such as 5-bromo-4-chloro-3-ind olyl-.beta.-D-glucopyranoside, and a second phosphatase substrate, such as 6-chloro-3-indolylphosphate or 5-bromo-6-chloro-3-indolylphosphate. In this embodiment, staphylococci in a sample will produce metabolites that will react with the phosphate substrate in the medium to pr oduce colonies having red to red-violet color while other bacteria in the sample will produce .beta.-glucosidase that will react with the glucopyranosidase substrate in the medium and produce colonies having a blue color. In a particularly preferred embodiment of this inventi on, a thin film culture plate device, such as a PETRIFILM culture plate device, is prepared using a dry culture medium containing sele cted inhibitors, a first glucopyranoside substrate and a second phosphate substrate. When the thin film culture plate device is inoculated w ith a sample and then incubated for a sufficient period of time, staphylococci in the sample will produce colonies on the thin film device ha ving a red color and other bacteria in the sample will produce colonies on the device having a blue color. |
| priorityDate | 1994-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.