http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2170967-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1996-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c146be26d256078408eb5c1aad7ca666 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b5a28b7b824950c7610b63a197464e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a79aeda92503cf1f9e84a4afbc23d28a |
| publicationDate | 1996-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2170967-A1 |
| titleOfInvention | Sample processing method for whole blood |
| abstract | Methods for processing whole blood for nucleic acid analysis of microorganisms which may be present are provided. The methods eliminate inhibitors which interfere in particular with enzymatic nucleic acid reactions and are also compatible with conventional culturing techniques. It has been found that selective lysis of red blood cells with certain lysis buffers, followed by centrifugation at 5,000-15,000 xg for 5-30 min. and washing with water, saline, physiological buffers, or buffers compatible with nucleic acid analysis removes inhibitors of nucleic acid reactions which are present in whole blood or are introduced by reagents used in the sample processing protocol. The method therefore allows nucleic acid analysis of large samples of whole blood without interference from inhibitors, thereby improving the sensitivity of the nucleic acid analysis. |
| priorityDate | 1995-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.