http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2168647-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_960be05712107f2fcbc5bca682785da8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e68893abe2a54dd4879eb9dc93c2c6e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-71 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-71 |
| filingDate | 1994-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54c5e2293f7dc3970bb78a5c5dcdcbcb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad7830b0bb9ea470ead38f7c6c956caf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_283cf3b89742ee994de96ce2daaae91a |
| publicationDate | 1995-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2168647-A1 |
| titleOfInvention | Monogenous preparations of cytotoxic conjugates |
| abstract | Monogenous preparations of conjugates of fibroblast growth factors (FGF) with cytotoxic agents are provided. Substantially all of the cytotoxic conjugates in the resulting monogenous preparation contain the same molar ratio of cytotoxic agent to polypeptide reactive with an FGF receptor. The conjugates are produced by chemical conjugation and also by expression of DNA encoding the conjugate to produce fusion proteins. The chemical conjugates are prepared by reacting FGF, which has at least one of the reactive cysteines replaced or deleted, with purified mono-derivatized saporin or with saporin that has been modified at or near one terminus by addition of a cysteine residue or replacement of a residue with cysteine. The resulting preparations are substantially monogenous. Fusion proteins are produced by expressing DNA constructs encoding muteins of an FGF in which one or more of any reactive cysteines have been deleted or replaced linked to DNA encoding a cytotoxic agent are also provided. The DNA constructs are expressed in host cells to produce monogenous preparations of cytotoxic FGF, conjugates. In embodiments in which the FGF portion of the conjugate has been modified so that there are no cysteines available for reaction, the resulting compositions are free of aggregates. The monogenous preparations of conjugates provided herein are potent cytocidal agent to cells bearing the FGF receptor. |
| priorityDate | 1993-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1017.