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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N-
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 1995-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00942a9caf9ca52062c6eee0902eb54a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1997fa0e7eda908cf7980b865158cb92
publicationDate 1996-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2161391-A1
titleOfInvention Stable transformation
abstract The invention provides a technique for stably inserting foreign genes into conifer cells. The cells to be transformed are in an undifferentiated state such that embryos are likely to regenerate from a single cell. Because of the use of this approach, the probability that the stably transformed plants will be chimaeric is low or non-existent. Conifer embryogenic tissue may be transformed subjected to extremely toxic levels of selection agent after insertion of foreign genes wherein the foreign genes include genes protective against the selection agent and wherein only stably transformed embryogenic tissue grows on the medium, and non-transformed tissue does not grow. The selected tissue may be used to produce transgenic plants. In preferred embodiments the cells to be transformed are maintained on a medium having concentrations of inorganic ions within a prescribed range.
priorityDate 1995-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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