http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2159081-C
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
filingDate | 1994-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2000-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ac18a7d15f21c4074f7c3c5d31be921 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_983a900d2983f6164d1800e095493bda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93707e7e337f419a97b793fc16dab90a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_216b18fc40ff376e7f986b514ab434ed |
publicationDate | 2000-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2159081-C |
titleOfInvention | Dinucleotide restriction endonuclease preparations and methods of use |
abstract | The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention. In addition, the present invention is directed to DNA sequences encoding a novel restriction endonuclease (designated R. Cvi JI) and variants thereof as well as to methods and materials for production of the same by recombinant methods. A bacterial host cell transformed with DNA encoding R. Cvi JI is also disclosed as well as methods for expressing R. Cvi JI in the bacterial host system and subsequent materials and methods for purifying the enzyme. |
priorityDate | 1993-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 676.