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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5120915c2421e046257f18b7035d6bf3
classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-813
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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535
filingDate 1993-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cf19a0e9803ee8b807b5f5a10187acc
publicationDate 1994-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2157177-A1
titleOfInvention Method and kit for isolation and purification of enzyme-antibody conjugates
abstract A process is described for isolating an enzyme-antibody conjugate, wherein the enzyme is horseradish peroxidase or alka-line phosphatase, from an aqueous mixture of said conjugate and unconjugated enzyme. The process involves contacting the mix-ture with a water insoluble stationary phase having the Ni2+ ion chelated thereto and binding said conjugate to the stationary phase. The phase containing bound conjugate is then washed to remove unbound enzyme. Thereafter the conjugate is eluted from the stationary phase and recovered in a form substantially free of the unconjugated enzyme as shown in the figure.
priorityDate 1992-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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