abstract |
By this invention, further plant medium-chain acyl-ACP thioesterases are provided, as well as uses of long-chain thioester-ase sequences in conjunction with medium-chain thioesterase sequences. In a first embodiment, this invention relates to particu-lar medium-chain thioesterase sequences from elm and Cuphea, and to DNA constructs for the expression of these thioesterases in host cells for production of C8 and C10 fatty acids. Other aspects of this invention relate to methods for using plant medium-chain thioesterases or medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells. As a further aspect, uses of long-chain thioesterase sequences for anti-sense methods in plant cells in conjunction with expression of medium-chain thioesterases in plant cells is described. |