http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2146162-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01a96e8bbea00eeaa0b50c2a0c000dba |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-31 |
| filingDate | 1993-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02105088696041b5654d42830b7d08c5 |
| publicationDate | 1994-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2146162-A1 |
| titleOfInvention | Affinity purification methods involving amino acid mimetics as elution reagents |
| abstract | Disclosed is a method of isolating a protein from a sample, involving (i) providing a first molecule which is capable of forming an affinity complex with the protein; (ii) contacting the sample with the first molecule under conditions which allow af-finity complex formation; (iii) isolating the complex; (iv) treating the complex with a second molecule, the second molecule mi-micking an amino acid residue of either the protein or the first molecule which is critical to the complex formation, so that the second molecule disrupts the complex; causing the release of the protein from the complex; and (v) isolating the protein. Accord-ing to one embodiment, the amino acid mimetic imidazole is used as a very gentle elution reagent to disrupt a protein A-antibody fusion protein complex, a technique which has general application for the isolation of antibodies or recombinant antibody fusion proteins. |
| priorityDate | 1992-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.