| abstract |
To reduce the difficulties of obtaining absorbance and emission bands that reduce the effects of background noise during gel electrophoresis when irradiating with a diode laser scanning, there is provided a method of identifying strands of DNA comprising the steps of marking the strands with fluorescent labels that emit light in a region of wavelengths including at least one wavelength within the infrared and near infrared region wherein the fluorescent label includes a chromophore having the formula: or or where X is (CH2)n; n = 4-10 or X is -CH2 - CH2 - 0 - CH2 - CH2 - 0 - CH2 - CH2 -; or wherein X consists of one of O or NH, Y consists of one of NCS or H; and R consists of one of H, NCS, CH2OH, CH2NCS, COOH, irradiating the strands with light having a wavelength within one of the infrared and near infrared regions; and detecting the light emitted from the fluorescent labels. There is also provided a dye having the above formula. |