Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7150f2fec2da2b08afde70802a1cd2d1 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 |
filingDate |
1993-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16642c93786918c0dfea90efa8207ec1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_233622b13c1124d3c1b289361f45592a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0106b617843a939f1aa621bfdb17b71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8397790b0750989e51d6516c47c0df58 |
publicationDate |
1993-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2127126-A1 |
titleOfInvention |
Chiral synthesis with modified enzymes |
abstract |
A method for modifying the specificity and/or efficiency of an enzyme, while retaining its catalytic activity, characterised in that it comprises: selecting an enzyme, the tertiary structure of which is substantially known or deduced; identifying at least one specificity and/or efficiency-related region; identifying or constructing unique restriction sites bounding the identified region in the DNA coding therefor; generating a DNA sequence which corresponds to at least a portion of the identified region, except that the nucleotides of at least one codon are randomized, or selecting as a substitute for at least a portion of the identified region an alternative such region, which may itself be similarly randomized; using the generated or substitute DNA sequence to replace the original such sequence; expressing the DNA including the generated or substitute DNA sequence; and selecting for a desired modification so that the DNA coding therefor may be isolated is disclosed. |
priorityDate |
1992-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |