http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2069523-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6893 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-30 |
| filingDate | 1992-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d68a66669ff2bf68fd8f10c4779f7b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1686b02b5848bf49281c3dd65a17cc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d1ecfbbeba371527455d42f81712b21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33c4fe9568dfdbffecb07cd450d80de2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c6fc2d491b018483280d0b643ede99a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_870d2be929177e3edd61b3a6b1227c5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f0ac9baa3071c9761720fae07f8c438 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2b6f33fe1191f3c7bb9e714bff51af8 |
| publicationDate | 1992-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2069523-A1 |
| titleOfInvention | Species-specific method for identifying infectivity of eimeria species |
| abstract | TITLE OF THE INVENTION SPECIES-SPECIFIC METHOD FOR IDENTIFYING INFECTIVITY OF ETMERIA SPECIES ABSTRACT OF THE INVENTION Unique methods are disclosed to determine the relative infectivity of each of multiple species of Eimeria in a multivalent live vaccine. Genomic deoxyribonucleic acid (DNA) is extracted from intestinal epithelia and mucosa of vaccinated birds and amplified using the polymerase chain reaction (PCR). The reaction allows efficient amplification of fragments from every small subunit ribosomal RNA (ssrRNA) gene in the target pool. Unique species-specific hybridization probes complimentary to sequences within the heterogeneous pool of PCR products are used to quantitate the relative levels of each of the Eimeria species in the infected hosts. The same unique probes are used to identify and quantitate species specific ssrRNA sequences in total RNA extracted from intestinal epithelia and mucosa of vaccinated chickens. The same unique probes are also used to identify and quantitate species specific ssrRNA sequences in genomic DNA isolated from mixed oocyst population purified from decal material obtained from vaccinated chickens. |
| priorityDate | 1991-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 200.