http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2064754-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a68d2c6598b59e0db05d8f59f1d1a315 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10222 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-585 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-585 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 1990-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1996-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c892e8882ca76b0b09284c10b206615e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52e52ddecb5ce35e7adad9a67a2c0713 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b491232c8f473131005305da0831cd3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a03eb43d5c38aa21a01babc996e1c64 |
| publicationDate | 1996-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2064754-C |
| titleOfInvention | Method of preparing polypeptides in a cell-free translation system |
| abstract | Polypeptides are synthesized over a several hour period in a cell-free system containing an exogenous prokaryotic RNA polymerase, a DNA molecule which encodes a polypeptide of interest, and additional substrates. In the cell-free polypeptide synthesis method, a mixture which includes a eukaryotic cell extract capable of supporting in vitro translation, ATP, GTP, CTP, UTP, and amino acids is added to a vessel containing an ultrafiltration membrane barrier. Reaction substrates including ATP, GTP, CTP, UTP and amino acids are continuously delivered to the vessel at a rate that maintains their initial concentration in the vessel while the products of the process, including AMP, GDP, CDP, UDP, pyrophosphate, inorganic phosphate, and the polypeptide product are continuously removed from the vessel through the ultrafiltration barrier. Included in the reaction mixture is an exogenous prokaryotic RNA polymerase and a DNA molecule which encodes the polypeptide of interest and contains a promoter site specific to the exogenous RNA polymerase. |
| priorityDate | 1989-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 169.