| abstract |
Bioassays for pollutants or toxicants can be performed at any time of year, even out of season, using cryopreserved unfertilised eggs of the phylum Mollusca, such as oysters and molluscs. The eggs are cryopreserved in an aqueous medium that contains a cryoprotectant such as dimethyl sulphoxide in contact with cholesterol, which may be coated onto an ampoule or cryopreservation straw or tube. |