http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2058453-C
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6481 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate | 1990-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1999-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdd04a7dd964f595a9ad1125c081c25e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af31cb7d07c70da881e38a7c98c6d2de |
| publicationDate | 1999-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2058453-C |
| titleOfInvention | Chymosin recovery and purification |
| abstract | 2058453 9015865 PCTABS00003 This invention provides a method of recovering and purifying chymosin from fermentation used for microbial production of chymosin. The fermentation mixture is filtered to provide a filtrate containing the chymosin. The filtrate is first prepared by either adjusting it to a low pH, such as about 2 and/or adjusting the salt concentration of the filtrate to at least about 2M. The aqueous filtrate mixture is contacted with a phenyl-sepharose resin, which selectively binds the chymosin from the filtrate. The filtrate remaining after the contact with the phenyl-sepharose resin is discarded, and the phenyl-sepharose resin column is eluted with water or dilute salt solution to obtain substantially pure chymosin in a single elution step. |
| priorityDate | 1989-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.