| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f054f5b3b0d5aab29f82c13e7d28009e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b0c198757da18cf77dfbdcef00abb70 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate |
1990-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2000-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b60468bdd85d79d7513710409e32f489
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e986411e2b75ba54620d33c2f130362
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d99c131337fa41fa5ea439e3dd746526
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e67580a16cda7c39a094dfa7db123ad
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_100b2d630c84ac3d44c9843029cf3950
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c673666663e2113e6d2a93e99d6f731 |
| publicationDate |
2000-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2050601-C |
| titleOfInvention |
Production in bacteria and yeast of hemoglobin and analogues thereof |
| abstract |
Alpha subunits of hemoglobin are provided as a novel recombinant di-alpha globin polypeptide comprising the two alpha subunits connected directly via peptide bond or indirectly by a flexible aminoacid or peptide linker. Di-alpha globin may be combined in vivo or in vivo with beta globin and heme to form hemoglobin. Tetrameric human hemoglobin and di-alpha/beta 2 hemoglobin are produced in S. cerevisiae by three types of expression vectors: (1) two separate plasmids containing respectively alpha and beta globin genes expressed in diploid strains; (2) a single plasmid comprising alpha and beta globin genes expressed in either haploid or diploid strains; (3) a single plasmid containing di-alpha and beta globin genes expressed in haploid strains. Tetrameric form or separate subunits can be recovered from the soluble fraction. So, three types of hemoglobin-like molecules can be produced: di-alpha/ two beta, di-beta/ two alpha or di-alpha/ di-beta, with a long half-life. |
| priorityDate |
1989-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |