http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2040180-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_571613fd00837119087160305f071016 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-975 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-06086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505 |
| filingDate | 1991-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2001-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50703c699a6d3fc534dae607047197b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbcce59bf2af2c341044aa9863ee4261 |
| publicationDate | 2001-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2040180-C |
| titleOfInvention | Enzymatic process for the determination of beta-lactam antibiotics |
| abstract | An enzymatic process for the determination of .beta.-lactam antibiotics in a biological liquid, comprising the steps of (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R39, the .beta.-lactam antibiotic reacting with the enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) incubating the mixture obtained with a solution of a thioester substrate having the formula wherein R1 = benzoyl, phenylacetyl or N .alpha.-acetyl-L-lysyl, R2 = glycyl or D-alanyl, and R3 = hydrogen or methyl so as to form, by hydrolysis, a 2-mercaptoalkanoic acid having the formula in an amount proportional to the residual enzymatic activity, said incubation being further conducted in the presence of glycine ar of a D-amino acid; (3) determining the amount of 2-mercaptoalkanoic acid formed, and (4) comparing the determination of step (3) with a standard, to obtain the antibiotic concentration in the biological liquid. A test set for carrying out this process is also disclosed. |
| priorityDate | 1990-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 174.