Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-867 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6459 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1133 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21069 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
filingDate |
1989-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1999-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f58eb99f62a081266a4adaea4a5272d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6512b5f73dc7c4b366e5a5e27ef60d28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_459376f71d7eef9c78c1ac9a5054661a |
publicationDate |
1999-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2000604-C |
titleOfInvention |
Process for the activation of genetechnologically produced, biologically-active proteins expressed in prokaryotes |
abstract |
The present invention provides a process for the activation of gene-technologically produced, biologically active proteins expressed in prokaryotes after cell digestion by solubilisation under denaturing conditions and reducing conditions and subsequent reactivation under oxidising and renaturing conditions, wherein working is carried out at a protein concentration of 1 to 1000 µg./ml. and, between the solubilisation and the reactivation, a dialysis is carried out against a buffer with a pH value of from 1 to 4 containing 4 to 8 mole/litre guanidine hydrochloride or 6 to 10 mole/ litre urea. |
priorityDate |
1988-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |