| abstract |
Substantially pure basic fibroblast growth factor (bFGF), a 146 amino acid residue polypeptide, is produced. The amino acid residue sequence of bFGF is disclosed as well as a DNA chain encoding the polypeptide. By appropriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform dells, synthetic bFGF can be obtained from transformed cell lines, both prokaryotic and eukaryotic. bFGF is a mitogenic factor for a wide variety of normal diploid mesoderm-derived and neural crest-derived cells. |