| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a89dfc566576a2eef71cfb4171cdbaf1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a61f538610b385bcea910799cf29afe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_800c1bffb5f33447d0dcbcff4a62f035
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5b1a92d2019a3eefe95cb9a60667aa51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_77551a0fe3747ff3f2776a31a0dca038 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-808
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-805 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44721
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44726
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-6512
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-6561
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65586
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D405-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D471-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6512
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B11-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D471-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-655
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D487-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6561
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6558
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D405-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D407-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate |
1987-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1999-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a15da17ffb1f29df9bb640ed289fd1af
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3a0c7c491e102df91b4903284f40aaa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40a54d44ec62fa5d8ad8fa902604a784
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2fea4e30c0808978aab62426efd55fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd1a8880c898358019dd9eb4da8ca6c2 |
| publicationDate |
1999-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-1340806-C |
| titleOfInvention |
Method, system and reagents for dna sequencing |
| abstract |
A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments. The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method. |
| priorityDate |
1986-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |