abstract |
Abstract A staining system for acid-fast bacilli which includes a primary stain and a secondary stain which have been used at an ambient temperatures. The primary stain includes basic fuchsin, ethyl alcohol, isopropyl alcohol, phenol and water. The secondary stain includes methylene blue, ethyl alcohol, potassium hydroxide, glycerol, glacial acetic acid, polyvinylpyrrolidone and water. |