http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1314254-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f8565c8b0315a5c1678dca65ddfbc47a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 |
| filingDate | 1987-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1993-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f26d5192cc7327bbbcff8b6e790bfd8 |
| publicationDate | 1993-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-1314254-C |
| titleOfInvention | Enzymatic membrane method for the synthesis and separation of peptides |
| abstract | ABSTRACT The present invention overcomes problems of precipitating an intermediary complex and/or rapidly deactivating enzymes associated with known methods. It provides a membrane method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the uncharged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively "pulled" across the membrane. An enzymatic conversion of the uncharged species utilizing an esterase such as aminoacylase I is disclosed. Copermeating reactants can be separated from the product mixture and returned to the reaction mixture. Also, the ion-rejection membrane can be utilized to resolve enantiomers of racemic carboxylic acids including D,L-amino acids. |
| priorityDate | 1987-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.