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publicationDate 1992-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-1303494-C
titleOfInvention Simultaneous enzyme immunoassay for detecting antigen and/or antibody in humans
abstract ABSTRACT OF THE DISCLOSURE A solid-phase immunoassay is provided for determination of members of an immunological pair of such antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or "spike" of the binding pair to the test sample prior to incuba-tion of the test sample in the presence of a solid-phase ab-sorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.
priorityDate 1987-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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