| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ac661be0b9a884290451af2b1432123 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-825
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S930-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-808 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0089
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-73 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-73
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-27
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-485
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61 |
| filingDate |
1984-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1989-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53539ea47bb975c018c6268498c96466
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdfa4ad4ed5df93ea54bf084884beff7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7808ea8609564fafb367c9a1dee6994
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bc4bfee378dc984501afddc86287933
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ea9906a38fdcfd72d7539590e4aaf13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_730a7ffd730f599fb1b5e6f4b5793fe3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_306d0ece9eac1dc400af809b74a4dc44 |
| publicationDate |
1989-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-1254527-A |
| titleOfInvention |
Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods |
| abstract |
EXPRESSION VECTORS FOR ENHANCED PRODUCTION OF POLYPEPTIDES, PLASMIDS CONTAINING THE VECTORS, HOSTS CONTAINING THE PLASMIDS, PRODUCTS MANUFACTURED THEREBY AND RELATED METHODS ABSTRACT OF THE DISCLOSURE An improved vector upon introduction into a suitable bac-terial host containing the thermolabile repressor CI ren-ders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene in-serted into the vector and production of polypeptide en-coded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator PLOL from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifi-able trait which is manifested when the vector is present in the host cell. |
| priorityDate |
1983-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |