http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1225609-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f1ebf89b1ed6362db9fc10ab0a3206de |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-55 |
| filingDate | 1984-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1987-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcab6f148ac95825b8f716b2507ec3a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9641dd277887065f7daf5f9e09e8d1ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6e75160fd3819c277bffdcac4e1e09c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e66d9caaba0d5e5aad031c75e659d33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b65b18613663d7053ef4503cae956b |
| publicationDate | 1987-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-1225609-A |
| titleOfInvention | Process for the preparation of a bacterial clone producing human .alpha .-1-antitrypsine |
| abstract | PRECISION OF DISCLOSURE: The present application relates to a method of preparation of a bacterial clone producing the alpha. 1-antitrypsin human. This process is characterized in that one extracts and purifies total messenger RNA from human liver, enriches these messengers in molecules coding for alpha. 1-antitrypsin human, in vitro synthesis of A DN is carried out double stranded from this messenger fraction enriched, we enrich the preparation of complementary AD double strand in molecules of size corresponding to that of A DN - AT, we add cohesive ends to this preparation of complementary double stranded DNA enriched in DNA -A T, this enriched fraction por-both cohesive ends with a plasmid vector carrying complementary cohesive ends to obtain hybrid molecules, we transform host bacteria Escherichia Coli by these hybrid molecules, we select among these transformed bacteria those which inserted a fragment of human A DN so as to obtain a bank of clones, the clones carrying the A DN are isolated from this bank -A T by characteristic screen of the alpha. 1-antitrypsin human, we identify among these clones those which express the alpha. Human 1-antitrypsin. |
| priorityDate | 1983-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 126.