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filingDate 1982-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1985-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85e91490bbe48edb3541fa6d42880a0f
publicationDate 1985-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-1185549-A
titleOfInvention Method for the preparation of an improved mutant strain of bordetella bronchiseptica useful as a live attenuated vaccine for prophylaxis of b. bronchiseptica infection
abstract Abstract of the Disclosure: The invention provides a method for the preparation of a new mutant strain of Bordetella bronchiseptica having a plurality of hereditary chromosomal markers comprising at least temperature-sensitivity and urease negativity. In particular, the invention provides a phase I mutant strain of B. bronchiseptica having excellent immunity and high safety, which is useful for the preparation of a live attenuated vaccine for prophylaxis of B. bronchiseptica infection, and also a live attenuated vaccine prepared there-from. The mutant strain is obtained by applying a mutagen to a strain of B. bronchiseptica to provide a mutant strain having temperature sensitivity and urease negativity, culturing the mutant strain, picking up a cell growth with a relatively small colony, and repeating the procedure of picking up a cell and culture thereof to convert it to a phase I organism having capsular antigen and which is hemolytic.
priorityDate 1981-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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