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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-09
filingDate 1983-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1985-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2b7fa7fff18ae8e626a1ab7b0dd037d
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publicationDate 1985-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-1183477-A
titleOfInvention Method for the preparation of glucosyltransferase
abstract METHOD FOR THE PREPARATION OF GLUCOSYLTRANSFERASE ABSTRACT OF THE DISCLOSURE A method of preparing a purified glucosyltransferase (GTF) for use in immunization against dental caries, which method comprises: culturing a Streptococcus mutans in a medium contain-ing glucose and dialyzable nutrients to form a mixture of culture cells and supernatant; recovering the supernatant by the removal of the culture cells; admixing the recovered supernatant with a water-insoluble, polymerized polysaccharide as solid particulate material for a period of time, to provide a GTF, solid particulate complex; recovering the GTF complex in solid particulate form by filtration; washing the solid GTF complex to remove unbound GTF and medium component; removing the GTF from the solid particulate material by a denaturing solvent; recovering the water-insoluble particulate material for reuse in the method; and recovering the GTF from the water-insoluble polysaccharide and purifying the recovered GTF.
priorityDate 1982-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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