http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1179928-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4dae7331034b6b26116b7c2f7acb115 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1982-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1984-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9906bfea86204d60d3afe76ffc0da653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_283c55ace8b80d28fe73284fbc175d2a |
| publicationDate | 1984-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-1179928-A |
| titleOfInvention | Nucleic acid spot hybridization of dna from eucaryotic cells and biological fluids |
| abstract | A method of detecting a specific DNA sequence in eucaryotic cells or a biological field using nucleic acid spot hybridization comprising the steps of contacting eucaryotic cells or a biological fluid with a support material capable of binding DNA, lysing the cells (when present) and denaturing the DNA so that the DNA present is immobilized on the support material, treating the support-material-immobilized DNA with a detectably-tagged DNA or RNA probe that is capable of forming a doublestranded structure with the specific DNA sequence, and detecting the tagged probe to determine the presence of double-stranded structure, which indicates the presence of the initially sought specific DNA sequence. Preferred support material is nitrocellulose, to which cells, single-stranded, and double-stranded DNA all adhere, but to which double-stranded DNA adheres most strongly. |
| priorityDate | 1981-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.