http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1174151-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f055bf58f0b16d53127fb8e2ab912b05 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-877 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate | 1981-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1984-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7120633f7e39084ad87f980821e621e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea192cc2a11e9c18be3e3c5bea35364d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e69a2a78eb5ffbef3c10a058d23a3a66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c618ddca0ab00f40cae0b536f7f7d35f |
publicationDate | 1984-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-1174151-A |
titleOfInvention | Method for the estimation of n-acylated primary aromatic amines |
abstract | ABSTRACT A method for the estimation of an anilide in which the anilide is first hydrolysed enzymatically to an aniline and then the quantity of the aniline produced is estimated spectrophoto-metrically, preferably colorimetrically. The hydrolysis of the anilide may be catalysed by any enzyme of the type, EC 3.5.1.13, known as aryl acylamidases. Preferably enzymes isolated from the cells of Pseudomonas fluorescens ATCC 39005 or Pseudomonas putida ATCC 39004 are employed. The aniline may be analysed, for example, by conversion to an indamine, an indophenol or an indoaniline, followed by colorimetric analysis of the coloured quinone-type compound produced. This conversion may take place in the presence of an oxidising agent, such as a copper (II) salt, and/or a base, such as ammonia. A diagnostic kit to allow the routine use of the above method is also provided. In one embodiment of the kit, it comprises, (a) an aryl acylamidase, in solution or on a solid support, (b) an organic compound suitable for the conversion of the aniline to an indamine, an indophenol or an indoaniline, preferably in solution, and (c) a base; preferably in solution. The kit may further comprise an oxidising agent, again preferably in solution. The above method of analysis and diagnostic kit may be particularly useful in the estimation of anilide drugs, such as paracetamol, dissolved in biological fluids. |
priorityDate | 1980-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 140.