http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1144051-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_87913ce8a05bad3cbd18ddeb65a67dbd |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-61 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-61 |
filingDate | 1981-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1983-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d3d5b1096bc870de857f77ab15cb8b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ca8924bca1d5dbb37dc29b104f4169b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fff604c867dd272835bf65c43c0795e0 |
publicationDate | 1983-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-1144051-A |
titleOfInvention | Method for the analysis of triglycerides |
abstract | ABSTRACT A method for the analysis of triglycerides in a biological fluid which comprises; (a) treating the bio-logical fluid to remove endogenous glycerol and pyruvate by combining with the fluid, as a first component, adenosine triphosphate (ATP), enzyme glycerol kinase (GK) and a metal cation activator for the GK, as a second component, enzyme pyruvate kinase (PK) in the presence of substrate phosphoenol pyruvate (PEP) and an enzyme PK activator, and as a third component, glutamate and enzyme alanine aminotransferase (GPT); (b) enzymatically hydrolyzing the triglycerides in the resulting fluid free of glycerol and pyruvate by com-bining the fluid with a mixture comprised of a microbial lipase, a surfactant selected from polyoxyethylene (POE) alkylamides, POE esters of fatty acids, POE mercaptans, POE alkylamines, POE polyol esters, POE acetylenic glycols and POE phosphate esters, and an alkaline earth metal cation, in the form of a salt; and (c) determining the amount of gly-cerol produced from the enzymatic hydrolysis. The method disclosed obviates the need for parallel blank correction. |
priorityDate | 1980-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 697.