http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1129316-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-805 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 |
filingDate | 1979-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1982-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a16637d51cff9cf216906bad3c6d0545 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa7b03f186424facc6c2d25aef6dc635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4304f6d9b93234209e54ec4b43dfb39 |
publicationDate | 1982-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-1129316-A |
titleOfInvention | Process for the quantitative enzymatic determination of adp |
abstract | Abstract In a process for the quantative enzymatic deter-mination of adenosine diphosphate (ADP) in aqueous solution by phosphorylation of the ADP with a kinase in the presence of a phosphorylation substrate therefor, with the formation of ATP and a dephoaphorylated sub-strate, such as galactose, formate, pyruvate or nucleoside diphosphate, the dephosphorylated substrate is reacted, optionally with the insertion of a further adjutant reaction, such as a decarboxylation, trans-amination or hexosidation, with NAD(P) in the presence of a dehgdrogenage and the NAD(P)H formed either measured directly or reacted with a tetrazolium salt in the presence of an electron carrier, with the formation of a formazane, which latter is then measured. A reagent suitable for carrying out this process contains a kinase, a phosphorylated substrate therefor different from ADP, a specific dehydrogenase for the dephosphorylated form of the substrate or for a trans-aminated, decarboxylated or hexosidised reaction product thereof, the co-enzyme of the dehydrogenase and a buffer, as well as optionally a tetrazolium salt, an electron carrier suitable therefor and optionally a surface-active agent. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5206146-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4767712-A |
priorityDate | 1978-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 343.