http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1071101-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07addebfc9446dc5a9a77692ebf4dac1 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-817 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-804 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-9453 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-94 |
filingDate | 1976-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1980-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fa44301b950e7b331bda716be4f3157 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_654149a80ed35fe600d6f0cf670fc219 |
publicationDate | 1980-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-1071101-A |
titleOfInvention | Radioimmunoassay method for the determination of cardiotonic glycosides |
abstract | ABSTRACT OF THE DISCLOSURE An improved, simplified radioimmunoassay method for the in vitro determination of cardio-tonic glycosides in unextracted blood serum involves the use of a reagent constituting a buffer solution containing a radioactive hapten for such glycosides. Packaged test kits for use in conveniencely carrying out the radioimmunoassay are also provided. The radioimmunoassay method involves the steps of mixing a sample of blood serum whose cardiotonic glyco-side content is to be determined with a reagent comprising an aqueous buffer solution containing a radioactive hapten for said glycoside, adding to the mixture an antiserum con-taining antibody capable of immunoreactivity with said glycoside and said hapten, incubating the resultant mixture at a temperature and for a sufficient period of time to pro-duce substantial equilibration of the antibody bound hapten and unbound hapten, adding a relatively thin strip of a membrane consisting essentially of an ion-exchange resin to the mixture and maintaining said membrane in contact therewith at a temperature and for a sufficient period of time to separate the unbound hapten from the antibody bound hapten, and determining the relative amounts of antibody bound radioactive hapten and unbound radioactive hapten. |
priorityDate | 1975-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.